Dictyostelium discoideum

Developmental cDNA Libraries of Full-length

(F-series: AF, CF, SF, and VF )
(H-series: AH, CH, SH, and VH )

Library construction

  1. Ax4 cells were used for library preparation.
  2. Total RNA was isolated from axenically growing cells (V), cells developed on nitrocellulose filter to at aggregation (8 hrs; A), slug (16 hrs; S) and early culmination stage (20 hrs; C ).
  3. cDNA was synthesized by oligo-capping method (Maruyama and Sugano, Gene 138:171-174, 1994, Suzuki et al., Gene 200:149-156, 1997) primed by oligo-dT with DraIII linker.
  4. . The cDNA was size-fractionated by agarose electrophoresis to yield >0.8 kb (for F-series) and >2 kb fraction (for H-series).
  5. The cDNA was uni-directionally ligated into the DraIII site of plasmid vector pME18SFL3 (Acc. No. AB009864).
  6. The ligated plasmid was introduced into E. coli DH10B.

(For more information including sequences around the cDNA insertion site, click here)

Clone and sequence information

If you have any interest in above libraries, please contact to Yoshimasa Tanaka.
Go to the cDNA Project home